Allen, Richard (2021). CIL:20803, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J01J98G8

To determine what happens to the CV during systole we observed the CV in a living cell and then fixed the cell as soon as the CV had opened to the outside. It was apparent that the CV membrane does not flatten into an empty sac but the CV membrane undergoes rapid tubule formation and collapses as a continuous mat of tubules that maintains its connection to the microtubular ribbons. These tubules are a uniform 40nm in diameter. TEM taken on 6/9/96 by R. Allen with Zeiss 10A operating at 80kV. Neg. 19,800X. Bar = 0.2µm. Adapted with permission from the J. Exp. Biol. 200:1737-1744, 1997. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. An unprocessed, high resolution version of this image (CIL:13113) is in the library and available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

• 2021

• Allen, Richard

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed

• Cell Image Library Group ID: 5664

• Paramecium multimicronucleatum

• Cell by organism
• Ciliated Protist
• Contractile vacuolar membrane
• Cytoplasmic microtubule
• Eukaryotic cell
• Eukaryotic Protist
• Integral to contractile vacuolar membrane
• Intrinsic to contractile vacuolar membrane

• Contractile vacuole organization
• Cytoplasmic microtubule organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 20803

Doi: https://doi.org/10.6075/J01J98G8

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL20803

Reference

• J. Exp. Biol. 200:1737-1744, 1997 https://www.ncbi.nlm.nih.gov/pubmed/?term=9319645

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2022-08-12