Cazares, Victor; Chao, Luke; Galbraith, James A.; Galbraith, Catherine G. (2021). CIL:36797, Drosophila melanogaster, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0W66JF9

The first image in a z-series of a structured illumination image (SIM) of microtubules in a Drosophila S2 cell. The red channel is the diffraction limited image and the green channel is the SIM image. The maximum projection of both z-series are available as CIL 36147 Note the two-fold increase in spatial resolution in the SIM image compared to the diffraction limited image. Images were collected as part of the Marine Biological Laboratory Neurobiology Course, Summer 2011.

• 2021

• Cazares, Victor
• Chao, Luke
• Galbraith, Catherine G.
• Galbraith, James A.

Cells were plated on Con-A coated coverslips, chemically fixed, and labeled with an anti-tubulin primary antibody and an Alexa Fluor 488 secondary antibody. Images were collected on a Zeiss Elyra Super-resolution microscope by Chris Rieken

Preparation: formaldehyde fixed tissue; glutaraldehyde fixed tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: structured illumination microscopy (SIM)
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: primary antibody plus labeled secondary antibody; Alexa Fluor 488
Processing history: maximum projection

• Cell Image Library Group ID: 9555

• Drosophila melanogaster

• Epithelial cell
• Microtubule

• Microtubule cytoskeleton organization
• Schneider S2

• data
• image

• No linguistic content; Not applicable

Samplenumber: 36797

Doi: https://doi.org/10.6075/J0W66JF9

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL36797

Other resource

• MBL Neurobiology Course 2011: http://www.mbl.edu/education/courses/summer/course_neurobio.html

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2022-08-12