Allen, Richard (2021). CIL:9704, Euplotes sp., cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0862FKR

A high resolution image of a dikinetid of Euplotes shows the transverse microtubular ribbon as well as a postciliary ribbon extending from the posterior basal body. Vesicles containing electron opaque bodies are typically found next to basal bodies. A peroxisome is also present. In this section a pattern of cross-hatched microtubules lies immediately under the cell surface membranes. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. TEM taken on 8/3/67 by R. Allen with Philips 200 operating at 60kV. Neg. 19,200X. The raw negative was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis. Additional information is available at (http://www5.pbrc.hawaii.edu/allen/).

• 2021

• Allen, Richard

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation

• Cell Image Library Group ID: 3503

• Euplotes sp.

• Cell by organism
• Ciliated Protist
• Cortical microtubule cytoskeleton
• Eukaryotic cell
• Eukaryotic Protist
• Microtubule basal body
• Mitochondrial inner membrane
• Mitochondrial outer membrane
• Symbiont-containing vacuole

• Cortical cytoskeleton organization
• Euplotes sp. from Carolina Biological Supply
• Mitochondrial organization
• Symbiont inclusion

• data
• image

• No linguistic content; Not applicable

Samplenumber: 9704

Doi: https://doi.org/10.6075/J0862FKR

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL9704

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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12