Jin, Seok Min; Lazarou, Michael; Wang, Chunxin; Kane, Lesley A.; Narendra, Derek P.; Youle, Richard J. (2021). CIL:13726, Mus musculus, fibroblast. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0P26WVK

Expression of WT PINK1-YFP (green) does not induce mitochondrial translocation of mCherry-Parkin (red) in PINK1 knock-out mouse embryonic fibroblasts (PINK1 KO MEFs). Transfected PINK1 KO MEFs were treated with DMSO for 3 hrs. Live cell imaging was performed on an LSM510 Meta (Carl Zeiss, Inc) with a 63x 1.4 NA oil differential interference contrast Plan Apo objective. Image contrast and brightness were adjusted in the LSM image browser (Zeiss). This image corresponds to Supplemental Figure S1c, top left row of J Cell Biol, 191: 933-942, 2010. Images in Supplemental Figure S1c include CIL#s 13725, 13726, 13727, 13728.

• 2021

• Jin, Seok Min
• Kane, Lesley A.
• Lazarou, Michael
• Narendra, Derek P.
• Wang, Chunxin
• Youle, Richard J.

Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EYFP; mCherryFP
Processing history: brightness and contrast adjusted
Data qualification: Raw;spatialmeasurements;intensitiesquantitation

• Cell Image Library Group ID: 7051

• Mus musculus

• Cytosol
• Fibroblast
• Mitochondrial inner membrane
• Mitochondrial outer membrane
• Mitochondrion
• Nucleus

• ATP binding
• C3HC4-type RING finger domain binding
• Calcium-dependent protein kinase activity
• Intracellular protein kinase cascade
• Magnesium ion binding
• Mitochondrion degradation
• Protein binding
• Protein K48-linked ubiquitination
• Protein K63-linked ubiquitination
• Protein phosphorylation
• Protein serine/threonine kinase activity
• Protein ubiquitination involved in ubiquitin-dependent protein catabolic process
• Response to stress
• Ubiquitin protein ligase binding
• Ubiquitin-protein ligase activity
• Zinc ion binding

• data
• image

• No linguistic content; Not applicable

Samplenumber: 13726

Doi: https://doi.org/10.6075/J0P26WVK

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL13726

Reference

• J Cell Biol, 191: 933-942, 2010. https://www.ncbi.nlm.nih.gov/pubmed/?term=21115803

Other resource

• BioStudies (previously JCB DataViewer): https://www.ebi.ac.uk/biostudies/studies/

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2022-08-12