CIL:12287, Homo sapiens, epithelial cell
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- Cite This Work
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Hotta, Azusa; Kawakatsu, Tomomi; Nakatani, Tomoya; Sato, Toshitaka; Matsui, Chiyuki; Sukezane, Taiko; Akagi, Tsuyoshi; Hamaji, Tomoki; Grigoriev, Ilya; Akhmanova, Anna; Takai, Yoshimi; Mimori-Kiyosue, Yuko (2021). CIL:12287, Homo sapiens, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0WW7GD9
- Description
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Behaviors of the microtubule anchoring factor RFP-LL5α (red) and microtubules (green) in living epithelial cells undergoing random migration. During the time-lapse imaging, the cells divided into two daughter cells. Cortical patches of RFP-LL5α appear when cells become attached to the glass substrate, but disappear when the cells become detached at the onset of cell division. The patches reappear soon after the cells adhere to the glass again. Overall findings from this publication showed that signaling from laminin-integrin associations plays a role in attaching microtubule plus ends to the epithelial basal cell cortex. This is the original data file for Video 4 from J Cell Biol (2010) 189 (5):901-917.
- Date Issued
- 2021
- Researchers
- Methods
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MCF-10Aeco cells expressing GFP-α-tubulin and RFP-LL5α were seeded on glass-bottomed dishes at a low density and cultured overnight. Time-lapse images of the cells were collected at 2.5-min intervals for 8 h 6 min using a DeltaVision Core Systemdriven by SoftWoRx software (Applied Precision) equipped with an IX70 microscope with a Plan-Apochromat 100× NA 1.40 oil immersion objective (Olympus), a cooled charge-coupled device camera (CoolSNAP HQ2; Photometrics) and a CO2 incubator (Tokai Hit Co., Ltd.). GFP, Ex, 470; Em, 525; RFP, Ex, 572, Em, 632.
- Technical Details
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Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: DsRed-monomerFP; EGFP
Data qualification: Raw - Series
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- Identifier
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Samplenumber: 12287
- Related Resources
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL12287
- Hotta A, Kawakatsu T, Nakatani T, Sato T, Matsui C, Sukezane T, Akagi T, Hamaji T, Grigoriev I, Akhmanova A, Takai Y, Mimori-Kiyosue Y. Laminin-based cell adhesion anchors microtubule plus ends to the epithelial cell basal cortex through LL5alpha/beta. J Cell Biol. 2010 May 31;189(5):901-17. https://doi.org/10.1083/jcb.200910095
- J. Cell Biol. 2010. 189(5):901-917 https://www.ncbi.nlm.nih.gov/pubmed/?term=0513769
- BioStudies (previously JCB DataViewer): https://www.ebi.ac.uk/biostudies/studies/
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- License
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License
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- UC Regents
- Copyright
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Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12