Allen, Richard (2021). CIL:9254, Coleps hirtus, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0FN15BZ

This high resolution micrograph may show important features involved in calcareous plate formation within the alveoli that underlie the plasma membrane in Coleps. Densities are seen on the inner alveolar membrane at its corners with septa. These densities are not known at this time in other ciliates and may be involved in calcareous platelet formation within the alveoli or in stabilizing the surface shape. Filamentous material fills the cytosolic space between two densities. Rough endoplasmic reticulum bears prominent ribosomes. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. TEM taken on 3/4/69 by R. Allen with Philips 300 operating at 60kV. Neg. 39,900X. The raw film was scanned with an Epson Perfection V750 Pro. This high resolution image is suitable for quantitative analysis.

Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

• 2021

• Allen, Richard

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation

• Cell Image Library Group ID: 3255

• Coleps hirtus

• Alveolar membranes
• Ciliated Protist
• Eukaryotic cell
• Eukaryotic Protist
• Plasma membrane
• Rough endoplasmic reticulum membrane

• Calcareous plate formation
• Calcium ion transport
• Cambridge Culture Collection, Cambridge, England, catalog # LB1656/1
• Membrane transport

• data
• image

• No linguistic content; Not applicable

Samplenumber: 9254

Doi: https://doi.org/10.6075/J0FN15BZ

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL9254

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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12