Perlstein, Ethan; Korostyshevsky, Daniel (2021). CIL:40415, Saccharomyces cerevisiae, CHC1 mutant. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0WS8S1F

Transmission electron micrograph of a representative sertraline-treated chc1 mutant cell. CHC1 encodes clathrin heavy chain, which trimerizes to form triskelia that coat vesicles. The vacuolar fragmentation phenotype is reversed by sertraline treatment. (8,000X). The untreated control image for the chc1 mutant is available as CIL 40416.

• 2021

• Korostyshevsky, Daniel
• Perlstein, Ethan

This group of images contains representative whole-cell images and high-magnification zooms of organelle structures of interest. All cells were grown in liquid rich media (YPD pH6.5) at 30C with aerated shaking, and cultures were sampled in mid-logarithmic phase. Treated cells were exposed to 60µM antidepressant sertraline (Zoloft®) for 45 minutes.

Preparation: glutaraldehyde fixed tissue; tissue in plastic embedment
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: osmium tetroxide
Processing history: unprocessed raw data

• Cell Image Library Group ID: 11312

• Saccharomyces cerevisiae

• CHC1 mutant
• Vacuole

• Organelle organization
• Regulation of vacuole organization
• Sertraline treatment

• data
• image

• No linguistic content; Not applicable

Samplenumber: 40415

Doi: https://doi.org/10.6075/J0WS8S1F

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL40415

Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License

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2022-08-12