Ammar, El-Desouky; Hogenhout, Saskia A.; Nault, L.R. (2021). CIL:25377, Maize mosaic virus, Peregrinus maidis, secretory cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J02V2F0T

Electron micrograph of Maize mosaic virus (MMV, Rhabdoviridae) in secretory cells from the principal salivary gland of the insect vector Peregrinus maidis (planthopper, Hemiptera, Delphacidae). MMV virions are bullet-shaped or bacilliform, 186-213 nm long and ca. 61 nm in diameter. In the principal salivary glands, MMV (arrows) buds through cytoplasmic membranes. Abbreviations: ics, intercellular space; SV, secretory vesicles. The accompanying image in this image group is unlabeled.

Methods: Dissected insect organs were processed for TEM by fixation in glutaraldehyde and osmium tetroxide and embedded in Spurr's medium. Thin sections were stained with uranyl acetate and lead citrate, and examined by a Philips-201 TEM.

Associated references:
Ammar, E.-D. and S. A. Hogenhout, S.A. (2008). A neurotropic route for Maize mosaic virus (Rhabdoviridae) in its planthopper vector Peregrinus maidis. Virus Research 131: 77-85.
Ammar, E.-D. and Nault, L.R. (1985). Assembly and accumulation sites of Maize mosaic virus in its planthopper vector. Intervirology 24: 33-41.

• 2021

• Ammar, El-Desouky
• Hogenhout, Saskia A.
• Nault, L.R.

Preparation: osmium tetroxide fixed tissue; glutaraldehyde fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: detection of electrons; illumination by electrons
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: uranyl salt; lead salt
Processing history: recorded image
Data qualification: Raw;spatialmeasurements

• Cell Image Library Group ID: 7996

• Maize mosaic virus
• Peregrinus maidis

• Secretory cell
• Vesicle

• Interspecies interaction between organisms
• Viral transmission by vector

• data
• image

• No linguistic content; Not applicable

Samplenumber: 25377

Doi: https://doi.org/10.6075/J02V2F0T

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL25377

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2022-08-12