Allen, Richard (2021). CIL:7590, Halteria grandinella, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J08C9V82

High resolution image of Halteria fixed in cacodylate-buffered fixative and stained. The round, dark bodies, possibly pigment granules, which were transparent in other preparations are electron-opaque following this protocol. The alveoli are flattened and inconspicuous. There is little evidence of an epiplasm. TEM taken on 11/8/68 by R. Allen with Philips 300 operating at 60kV. Neg. 12,500X. The raw film was scanned with a Nikon Coolscan 9000ED. This image is suitable for quantitative analysis.

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness.

Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

• 2021

• Allen, Richard

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation

• Cell Image Library Group ID: 3458

• Halteria grandinella

• Cell by organism
• Ciliated Protist
• Eukaryotic cell
• Eukaryotic Protist
• Food vacuole
• Macronucleus
• Pigment granule

• Carolina Biological Supply Co., NC, U.S.A.
• Cellular pigmentation
• Digestion
• Macronucleus organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 7590

Doi: https://doi.org/10.6075/J08C9V82

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL7590

CC0 1.0 Universal Public Domain Dedication

Creative Commons Public Domain Dedication

Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.

Constraint(s) on Use: This work may be used without prior permission.

Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12