Boassa, Daniela (2021). CIL:40249, Canis lupus familiaris, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0X63N2S

A single frame from a time-lapse animation of FlAsH-labeled MDCK cells expressing Cx43-4C309/337 (green) showing their rearrangement and fate during and after mitosis. In the time-lapse series, which can be accessed using the link provided to the Cell Centered Database, FlAsH fluorescence excitation z series along with a transmitted DIC image were recorded every 6 min for a total duration of about 5 h. 3D volume reconstructions from confocal image stacks over time are displayed at a rate of two frames per minute. From Supplemental movie 2 from Boassa et al. (2010) Traffic. PMID 20716111.

• 2021

• Boassa, Daniela

MDCK cells stably expressing two internal 4C domains in the C-terminus (FLNCCPGCCME)-tagged Cx43 (Cx43-4C309/337) were labeled for 1 h at 37 degrees C with 180 nM FlAsH-EDT2/12.5 uM EDT in Hanks balanced salt saline (HBSS). Free and non-specifically bound FlAsH was removed by washing with 2,3-dimercapto-1-propanol (BAL, 500 uM, 20min at 37 degrees C in HBSS). Time-lapse imaging was conducted using an Olympus FluoView1000 confocal microscope equipped with a temperature controlled chamber (at 37 degrees C) and a 60X 1.42 NA objective. Medium: Opti-MEM supplemented with 5% FBS covered with a glass coverslip.

Preparation: osmium tetroxide fixed tissue; glutaraldehyde fixed tissue
Relation to intact cell: living tissue; dispersed cells in vitro
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP
Data qualification: Processed

• Cell Image Library Group ID: 12252

• Canis lupus familiaris

• Connexon complex
• Epithelial cell

• Connexin binding
• Gap junction assembly
• MDCK (NBL-2)

• data
• image

• No linguistic content; Not applicable

Samplenumber: 40249

Doi: https://doi.org/10.6075/J0X63N2S

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL40249

Previous version

• Item in Cell Centered Database, UC San Diego Library Digital Collections: https://doi.org/10.6075/J0J10300

Reference

• PubMed article: https://www.ncbi.nlm.nih.gov/pubmed/?term=20716111

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2022-08-12