Allen, Richard; Schroeder, C. (2021). CIL:13130, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J02F7MK8

High resolution image of quick-freeze deep-etch view of a radial arm showing a collecting canal surrounded with smooth spongiome as well as a few decorated tubules. However several larger vesicles lie next to the radial arm containing a lumen resembling the contractile vacuole. These vesicles appear to be rounded decorated tubules which have been transformed into vesicles or have not yet tubulated and become part of the decorated spongiome of the mature radial canal. Although we cannot be certain without further study we believe this may represent a dividing cell as we do know that the radial arms of the dividing cell, both their smooth and decorated spongiomes, shorten as the new CVCs develop (Allen et al., J. Cell Sci. 96:469-475, 1990; Fok et al., J. Eukaryot. Microbiol. 49:185-196, 2002. The P-face of these vesicles has a large number of IMPs like that seen in collecting canals. The highly porous E-face of several vesicles indicates that these vesicles are indeed products of the decorated spongiome. TEM taken on 6/22/88 by C. Schroeder with Zeiss 10A operating at 80kV. Neg. 9,780X. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

• 2021

• Allen, Richard
• Schroeder, C.

Preparation: freeze_fracture/freeze_etch
Relation to intact cell: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: electron density
Parameter imaged: elastic scattering of electrons; electron density; elevation
Source of contrast: differences in deposition of metal shadow
Visualization methods: transmission electron microscopy (TEM)
Processing history: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements

• Cell Image Library Group ID: 9798

• Paramecium multimicronucleatum

• Cell by organism
• Ciliated Protist
• Contractile vacuolar membrane
• Contractile vacuole
• Eukaryotic cell
• Eukaryotic Protist
• Integral to contractile vacuolar membrane

• Contractile vacuole organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 13130

Doi: https://doi.org/10.6075/J02F7MK8

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL13130

Other resource

• Paramecium and Other Ciliates: Richard Allen's Image Collection. Pacific Biosciences Research Center. University of Hawaii at Manoa: https://www5.pbrc.hawaii.edu/allen/

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2022-08-12