Ghosh, Rajarshi; Woodcock, Christopher (2021). CIL:8045, Mus musculus. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0NC6063

Fluorescence recovery after photobleaching (FRAP)kinetics of MeCP2 were recorded using a mouse fibroblast cell line stably expressing MeCP2-GFP. Live cells were imaged using a Zeiss LSM 510 meta confocal microscope with fluorescence excitation at 488 nm and a 63x plan-apo oil lens. The image series has 2 pre-bleach shots, one immediately after bleaching a 1 micrometer diameter circle of pericentromeric heterochromatin, followed by 107 images at 2 sec intervals.

• 2021

• Ghosh, Rajarshi
• Woodcock, Christopher

Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: quantity of GFP-protein
Visualization methods: Green fluorescent proteins from Anthozoa
Processing history: unprocessed raw data

• Cell Image Library Group ID: 1515

• Mus musculus

• Nuclear chromatin

• Mobility of nuclear protein

• data
• image

• No linguistic content; Not applicable

Samplenumber: 8045

Doi: https://doi.org/10.6075/J0NC6063

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL8045

Reference

• Ghosh RP, Horowitz-Scherer RA, Nikitina T, Shlyakhtenko LS, Woodcock CL. MeCP2 binds cooperatively to its substrate and competes with histone H1 for chromatin binding sites. Mol Cell Biol. 2010 Oct;30(19):4656-70. https://doi.org/10.1128/MCB.00379-10
• PubMed article: https://www.ncbi.nlm.nih.gov/pubmed/?term=18334558

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2022-08-12