Lam, Wai Yan; Rice, Marian (2021). CIL:27229, Fundulus heteroclitus, scale. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0PZ57SD

Collagen fibrils and mineralization in the external layer of Fundulus heteroclitus scale. The external layer is the second of three layers closest to the dermis of an elasmoid. It is composed of thin collagen fibrils about 30nm in diameter, which are organized in a loose meshwork. This layer is the first layer to be formed and mineralized. This mineralization is visible in the extracellular material surrounding the collagen fibrils where mineral deposits in the form of needle-like crystals identified to most likely be hydroxyapatite can be found.

• 2021

• Lam, Wai Yan
• Rice, Marian

Fundulus heteroclitus scales were chemically fixed with 2.5% glutaraldehyde, 2% formaldehyde in 0.1M cacodylate buffer (pH 7.3), then post-fixed in 4% osmium tetroxide and stained en bloc in 1% uranyl acetate. The scales were then dehydrated in a graded series of ethanol and infiltrated with Spurr's resin. Thin sections of 70 nm were trimmed using a diamond knife and post-stained in uranyl acetate and lead citrate. This micrograph was imaged using a Phillips CM 100 transmission electron microscope at an accelerating voltage of 80 kV.

Preparation: glutaraldehyde fixed tissue; formaldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; dehydrated in ethanol
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt; lead salt
Processing history: unprocessed raw data

• Cell Image Library Group ID: 9597

• Fundulus heteroclitus

• Fibrillar collagen
• Mineralization in external layer
• Scale

• Extracellular matrix organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 27229

Doi: https://doi.org/10.6075/J0PZ57SD

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL27229

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2022-08-12