Microscopy product ID: 6347
2D Image
Description | Single tilt image at zero degress of tilt through a lamellar structure observed in HeLa cells transfected with mutant connexin 50, imaged using intermediate voltage electron microscopy, and labeled using the tetracysteine-ReHAsH system and fluorescence photoooxidation. The concentric double membrane layers of the aggregate appear to form ┐onion skin┐ sheets that do not form a closed structure along the long axis. The differential staining between the outside and inside is most likely due to impeded diffusion of DAB into the interior of these accumulations. Dark particles are 20 nm gold particles applied to the surface of the section to serve as fiducial marks for subsequent alignment. |
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Description | Tar file containing the raw and aligned tilt images, along with all supporting files from IMOD (beyers1a_*). Note that some of the tilt images were discarded. |
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Description | Animation through an aligned tilt series through a lamellar structure observed in HeLa cells transfected with mutant connexin 50, imaged using intermediate voltage electron microscopy, and labeled using the tetracysteine-ReHAsH system and fluorescence photoooxidation. Dark particles are 20 nm gold particles applied to the surface of the section to serve as fiducial marks for subsequent alignment. |
Reconstruction
Description | Single computed slice through a tomographic reconstruction of a lamellar structure observed in HeLa cells transfected with mutant connexin 50, imaged using intermediate voltage electron microscopy, and labeled using the tetracysteine-ReHAsH system and fluorescence photoooxidation. The concentric double membrane layers of the aggregate appear to form ┐onion skin┐ sheets that do not form a closed structure along the long axis. The differential staining between the outside and inside is most likely due to impeded diffusion of DAB into the interior of these accumulations. |
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Description | Tomographic reconstruction in IMOD format. |
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Description | Animation through the computed slices of a tomographic reconstruction of a lamellar structure observed in HeLa cells transfected with mutant connexin 50, imaged using intermediate voltage electron microscopy, and labeled using the tetracysteine-ReHAsH system and fluorescence photoooxidation. The concentric double membrane layers of the aggregate appear to form ┐onion skin┐ sheets that do not form a closed structure along the long axis. The differential staining between the outside and inside is most likely due to impeded diffusion of DAB into the interior of these accumulations. |
Scope And Content | This file was added on 2021-03-17. |
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- Cite This Work
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Beyer, Eric; Sosinsky, Gina; Crum, John; Berthoud, Viviana; Lichtensetin, Alexandra; Gaietta, Guido (2017). Microscopy product ID: 6347. In Cell Centered Database. UC San Diego Library Digital Collections. https://doi.org/10.6075/J0FJ2GK5
- Date Issued
- 2017
- Research Team Head
- Researchers
- Technical Details
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Female
Product type: SINGLE TILT. Microscopy type: IVEM. Instrument: JEOL 4000EX IVEM
- Funding
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National Eye Institute, National Institutes of Health
- Series
- Scientific Name
- Anatomy
- Topic
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- Language
- No linguistic content; Not applicable
- Related Resources
- Lichtenstein A, Gaietta GM, Deerinck TJ, Crum J, Sosinsky GE, Beyer EC, Berthoud VM (2009). The cytoplasmic accumulations of the cataract-associated mutant, Connexin50P88S, are long-lived and form in the endoplasmic reticulum. Experimental Eye Research, 88(3):600-609. PubMed: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2695785/; Experimental Eye Research. https://doi.org/10.1016/j.exer.2008.11.024
- Microscopy product 6347 at the Cell Centered Database: https://doi.org/10.7295/W9CCDB6347
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Creative Commons Attribution 4.0 International Public License
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- UC Regents
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Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2023-05-22