CIL:21996, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist
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- Collection
- Cite This Work
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Allen, Richard (2021). CIL:21996, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0ST7P17
- Description
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The plasma membrane of Paramecium multimicronucleatum seen in a freeze fracture replica. The two rows of IMPs can be seen on the P-face of the plasma membrane that connects the plasma membrane to the underlying margins of alveolar sacs. A gap 20nm wide composed of only the plasma membrane connects these two ribbons. The spent vacuole membrane will fuse with the membrane over this gap. Further interpretation of this image can be seen at Figure 4 of Chapter 7 at the link below. TEM taken on 6/3/75 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 12,750X. Bar = 0.5µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. An unprocessed, high resolution version of this image (CIL:12346) is in the library and available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
- Date Issued
- 2021
- Researcher
- Technical Details
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Preparation: freeze_fracture/freeze_etch
Relation to intact cell: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; elastic scattering of electrons
Parameter imaged: elastic scattering of electrons; electron density; elevation
Source of contrast: differences in deposition of metal shadow
Visualization methods: transmission electron microscopy (TEM)
Processing history: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film; Print from negative scanned for Photoshop
Data qualification: Processed - Series
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- Language
- No linguistic content; Not applicable
- Identifier
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Samplenumber: 21996
- Related Resource
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL21996
Source data
- License
- Copyright
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Creative Commons Public Domain Dedication
Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Constraint(s) on Use: This work may be used without prior permission.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12