CIL:40113, Drosophila melanogaster, embryonic cell
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- Collection
- Cite This Work
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Ellisman, Mark; Sosinsky, Gina; Jones, Ying (2021). CIL:40113, Drosophila melanogaster, embryonic cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0G44PPX
- Description
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Electron micrograph of a cultured Drosophila DL1 cell infected with flock house virus, prepared using chemical fixation followed by routine embedding for electron microscopy. This specimen was prepared as a control to compare different high pressure freezing protocols. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database.
- Date Issued
- 2021
- Researchers
- Methods
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Cell pellets were conventionally prepared for electron microscopy by incubation in 2% glutaraldehyde in 100 mM cacodylate approx 5 min) and then on ice for 30 minutes, followed by 1 percent osmium tetroxide in double distilled water for 1 hour and 2 percent UA in water overnight. Images were acquired using a JEOL4000EX IVEM, magnification: 30000.0; accelerating voltage: 80.0 keV.
- Technical Details
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Preparation: osmium tetroxide fixed tissue; glutaraldehyde fixed tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Parameter imaged: electron density
Data qualification: Processed - Series
- Scientific Name
- Anatomy
Formats
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- Language
- No linguistic content; Not applicable
- Identifier
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Samplenumber: 40113
- Related Resources
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL40113
- Item in Cell Centered Database, UC San Diego Library Digital Collections: https://doi.org/10.6075/J0MS3SK7
Source data
Previous version
- License
- Copyright
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Creative Commons Public Domain Dedication
Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Constraint(s) on Use: This work may be used without prior permission.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12