Woodcock, Christopher; Luda Shlyaktenko (2021). CIL:7120, Homo sapiens, neuron. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0542MJR

The protein MeCP2 binds to symmetrically methylated CpG dinucleotides and is thought to transmit the (generally) repressive signals encoded in DNA methylation patterns. Mutations in MeCP2 lead to Rett syndrome. Here, MeCP2-DNA complexes have been imaged by AFM (Digital Instruments Nanoscope) in air using human MeCP2 and an 828 bp DNA sequence containing several methylated CpGs. MeCP2 molecules appear as bright particles in AFM images. Volume measurements of the complexes suggest that MeCP2 monomers (circular particles) and dimers (larger elliptical particles) are present and suggest that MeCP2 binds DNA cooperatively. Other images in the grouped set show looped structures and large complexes containing multiple DNA strands and MeCP2 molecules. These configurations are likely promoted by the multiple independent DNA-binding domains of MeCP2 and relevant to the in vivo interactions between MeCP2 and chromatin.

• 2021

• Luda Shlyaktenko
• Woodcock, Christopher

Preparation: glutaraldehyde fixed tissue
Relation to intact cell: isolated subcellular component
Item type: recorded image
Imaging mode: microscopy; atomic force microscopy
Parameter imaged: specimen height
Source of contrast: specimen height
Processing history: unprocessed raw data

• Cell Image Library Group ID: 7765

• Homo sapiens

• Neuron
• Nucleus

• Rett syndrome
• Transcription regulation

• data
• image

• No linguistic content; Not applicable

Samplenumber: 7120

Doi: https://doi.org/10.6075/J0542MJR

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL7120

Reference

• Ghosh RP, Horowitz-Scherer RA, Nikitina T, Shlyakhtenko LS, Woodcock CL. MeCP2 binds cooperatively to its substrate and competes with histone H1 for chromatin binding sites. Mol Cell Biol. 2010 Oct;30(19):4656-70. https://doi.org/10.1128/MCB.00379-10
• PubMed article: https://www.ncbi.nlm.nih.gov/pubmed/?term=20679481

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2022-08-12