Microscopy product ID: 3603
Segmentation
Description | Manual segmentation of T tubule and SR membranes and mitochondria using IMOD 3.9.3 |
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Full resolution data file
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Description | Zip file containing the model file generated by IMOD (WT1_CCDB.mod) and measurements derived from them in IMOD (WT1_CCDB_IMODINFO.txt). |
Reconstruction
Description | Single computed slice from a tomographic reconstruction of a 500 nm section through the middle free wall of a cardiac myocyte from the left ventricle of an adult mouse (6-8 months of age), stained with potassium ferrocyanide to increase the contrast of membrane systems. |
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Full resolution data file
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Description | file contains TxBR (projective) generated tomographic volume of heart tissue from mouse left ventricle. Full volume: output.rec, trimmed volume:output.rec_sm, binned down volume: output.rec_bin2. Full resolution volume was used for segmentation. |
Down sampled data file
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Description | Downsampled version of reconstruction file in IMOD format (~2.0 Gb vs 16 Gb full resolution). Note that segmentation was performed on full resolution volume, available as one of the reconstructions available in the tar file through the Reconstruction download. |
Animation
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Description | Animation through the computed slices of a a tomographic reconstruction of a 500 nm section through the middle free wall of a cardiac myocyte from the left ventricle of an adult mouse (6-8 months of age), stained with potassium ferrocyanide to increase the contrast of membrane systems. |
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- Cite This Work
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Hoshijima, Masahiko; Hayashi, Takeharu; Thor, Andrea; Terada, Masako; Martone, Maryann; Ellisman, Mark H. (2017). Microscopy product ID: 3603. In Cell Centered Database. UC San Diego Library Digital Collections. https://doi.org/10.6075/J03B5ZWZ
- Creation Date
- Project: 2004-04-01.
- Date Issued
- 2017
- Research Team Heads
- Researchers
- Technical Details
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Male. Strain: S129
Product type: SINGLE TILT. Microscopy type: IVEM. Instrument: JEOL 4000 #1
- Series
- Scientific Name
- Anatomy
- Topic
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- Language
- No linguistic content; Not applicable
- Related Resources
- Hayashi T, Martone ME, Yu Z, Thor A, Doi M, Holst MJ, Ellisman MH, Hoshijima M (2009). Three-dimensional electron microscopy reveals new details of membrane systems for calcium signaling in the heart. J. Cell Science, 122:1005-1013. PubMed: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2720931/; Journal of Cell Science. https://doi.org/10.1242/jcs.028175
- Microscopy product 3603 at the Cell Centered Database: https://doi.org/10.7295/W9CCDB3603
Primary associated publication
Other version
- License
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Creative Commons Attribution 4.0 International Public License
- Rights Holder
- UC Regents
- Copyright
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Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2023-05-22