Hull, Barbara; Staehelin, Andrew (2021). CIL:11182, Bufo, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0JD4VMG

Freeze fracture replicas of zonula occludens junctions from the large intestine of a tadpole (upper) and intestine of a post-metamorphic toad (lower). The occluding junction appears as a meshwork of ridges on the P-face or a corresponding pattern of grooves on the E-face. Images originally published in Hull and Staehelin, J. Cell Biol. 68:688-704, 1976 and reprinted with permission as Figures 62 (upper) and 63 (lower) from Chapter 3 (Junctional Specializations) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. A PDF copy of the accompanying chapter is available on the ASCB's BioEDUCATE website.

• 2021

• Hull, Barbara
• Staehelin, Andrew

Preparation: freeze_fracture/freeze_etch
Relation to intact cell: isolated subcellular component
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: differences in deposition of metal shadow
Visualization methods: shadowing and plating
Data qualification: Processed

• Cell Image Library Group ID: 11071

• Bufo

• Epithelial cell
• Tight junction

• Cell junction organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 11182

Doi: https://doi.org/10.6075/J0JD4VMG

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL11182

Reference

• B E Hull, L A Staehelin; Functional significance of the variations in the geometrical organization of tight junction networks.. J Cell Biol 1 March 1976; 68 (3): 688–704. https://doi.org/10.1083/jcb.68.3.688
• PubMed article: https://www.ncbi.nlm.nih.gov/pubmed/?term=030707
• Wooster MS, Wrigglesworth JM. Modification of glyceraldehyde 3-phosphate dehydrogenase activity by adsorption on phospholipid vesicles. Biochem J. 1976 Dec 1;159(3):627-31. https://doi.org/10.1042/bj1590627

Other resource

• Fawcett, Don W. "The Cell." 2nd ed., W.B. Saunders, 1981, ISBN 0721635849. https://www.ascb.org/career-development/teaching/don-fawcetts-the-cell/

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2022-08-12