Wuehr, Martin; Mitchison, Timothy J. (2021). CIL:9512, Xenopus laevis. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0RJ4HN1

Frog (Xenopus laevis) egg arrested in metaphase of mitosis. Note the tiny metaphase spindle on the top left of the egg.

Eggs were fixed in 90% MeOH; 10% (H20 500mM EGTA) and stained for DNA with YoPro1 (green) and tubulin (red) with antibody B-5-1-2 directly labeled with Alexa 647. A single z slice was collected with a Zeiss LSM 710 using a 10 X 0.3NA objective. Lasers were 488 (DNA stain) and 633 (Tubulin stain).

• 2021

• Mitchison, Timothy J.
• Wuehr, Martin

Preparation: methanol fixed tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: YO-PRO-1; Alexa Fluor 647; labeled primary antibody
Processing history: unprocessed raw data

• Cell Image Library Group ID: 7118

• Xenopus laevis

• Condensed chromosome
• Spindle microtubule

• Meiotic metaphase II

• data
• image

• No linguistic content; Not applicable

Samplenumber: 9512

Doi: https://doi.org/10.6075/J0RJ4HN1

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL9512

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2022-08-12