Sengupta, Prabuddha (2021). CIL:9162, Homo sapiens, permanent cell line cell, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J08S4P30

Sialyl Transferase-EGFP transfected HeLa cells demonstrating the redistribution of the Golgi Apparatus to the Endoplasmic Reticulum upon treatment with Brefeldin A (BFA).
Images were collected as a 4D time series (z-stack over time). z-stacks of 48 individual timepoints are included in this collection of files as well as the movie SiEGFP_BFA.avi, which is the maximum projection of the z-stacks at the individual time points.

BFA was added according to the following protocol:
Timepoint1: Before addition of BFA 0seconds (8 Z-slices at 1 um apart)
Timepoint2: After addition of BFA 130seconds (8 Z-slices at 1 um apart)
Timepoint3: 238 seconds (11 Z-slices at 1 um apart)
Timepoint3 thru Timepoint48: Images captured every 5 seconds (11 Z-slices at 1 um apart at each time point)

Images were collected on a Carl Zeiss spinning disk confocal with a 63X 1.4 NA objective with 250 ms exposures at 5 s intervals.

• 2021

• Sengupta, Prabuddha

Preparation: unfixed tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: spinning disk confocal microscopy
Parameter imaged: fluorescence microscopy
Source of contrast: distribution of a specific protein
Visualization methods: EGFP
Processing history: unprocessed raw data

• Cell Image Library Group ID: 7926

• Homo sapiens

• Endoplasmic reticulum
• Epithelial cell
• Golgi apparatus
• Permanent cell line cell

• ER to Golgi vesicle-mediated transport
• HeLa

• data
• image

• No linguistic content; Not applicable

Samplenumber: 9162

Doi: https://doi.org/10.6075/J08S4P30

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL9162

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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12