Morselli, Eugenia; Mariño, Guillermo; Bennetzen, Martin v.; Eisenberg, Tobias; Megalou, Evgenia; Schroeder, Sabrina; Cabrera, Sandra; Bénit, Paule; Rustin, Pierre; Criollo, Alfredo; Kepp, Oliver; Galluzzi, Lorenzo; Shen, Shensi; Malik, Shoaib Ahmad; Maiuri, Maria Chiara; Horio, Yoshiyuki (2021). CIL:13917, Mus musculus, hepatocyte. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0XP73N4

Transgenic C57BL/6 mice expressing GFP-LC3 fusion protein, uninjected as control for injection with Resveratrol and/or spermidine. To avoid postmortem autophagy induction, dead mice were immediately perfused with 4% paraformaldehyde (wt/vol in PBS, pH 7.4). Tissues were then harvested and further fixed with the same solution for ≥4 h followed by treatment with 15% sucrose (wt/vol in PBS) for 4 h and with 30% sucrose (wt/vol in PBS) overnight. Tissue samples were embedded in Tissue-Tek OCT compound (Sakura) and stored at −70C. 5-µm-thick tissue sections were generated with a cryostat (CM3050 S; Leica), air dried for 1 h, washed in PBS for 5 min, dried at RT for 30 min, and mounted with Vectashield antifading medium. Confocal fluorescent images were captured using a confocal fluorescence microscope (TCS SP2; Leica) fitted with an Apochromat 40× 1.15 NA immersion objective. Images were acquired with a camera (DFC 350 FX 1.8.0; Leica) using LAS AF software (Leica) and processed with Photoshop (CS2; Adobe) software. Specifically, picture processing involved cropping of representative areas and linear adjustments of contrast and brightness and was performed using Photoshop (with equal adjustment parameters for all pictures); no explicit γ correction was used. Image: Figure 9C, bottom left panel (liver/Co), in Morselli et al. J Cell Biol 192: 615-629

• 2021

• Bennetzen, Martin v.
• Bénit, Paule
• Cabrera, Sandra
• Criollo, Alfredo
• Eisenberg, Tobias
• Galluzzi, Lorenzo
• Horio, Yoshiyuki
• Kepp, Oliver
• Maiuri, Maria Chiara
• Malik, Shoaib Ahmad
• Mariño, Guillermo
• Megalou, Evgenia
• Morselli, Eugenia
• Rustin, Pierre
• Schroeder, Sabrina
• Shen, Shensi

Preparation: formaldehyde fixed tissue; embedded tissue; cryostat-sectioned tissue
Relation to intact cell: sectioned tissue
Item type: recorded image; charge coupled device (CCD)
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP
Processing history: brightness and contrast adjusted
Data qualification: Processed;intensitiesquantitation

• Cell Image Library Group ID: 5404

• Mus musculus

• Autophagic vacuole
• Hepatocyte

• Autophagy

• data
• image

• No linguistic content; Not applicable

Samplenumber: 13917

Doi: https://doi.org/10.6075/J0XP73N4

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL13917

Reference

• J Cell Biol 192: 615-629 https://www.ncbi.nlm.nih.gov/pubmed/?term=21339330

Other resource

• BioStudies (previously JCB DataViewer): https://www.ebi.ac.uk/biostudies/studies/

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2022-08-12