Valerio-Santiago, Mauricio; Monje-Casas, Fernando (2021). CIL:13872, Saccharomyces cerevisiae. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0251GX2

Tem1-CAAX (green) is targeted away from spindle pole bodies in bfa1Δ cells. The CAAX motif from Ras2 targets proteins to the cytoplasmic side of the membrane. tem1Δ::GAL-UPL-TEM1 cells expressing eGFP-TEM1-CAAX from a CEN plasmid were grown on 2% raffinose/2% galactose and transferred to 2% glucose medium prior to image capture. A differential interference contrast (DIC) image is also shown (hidden). The tem1Δ::GAL1-UPL-TEM1 strain allows for the rapid, conditional depletion of Tem1. UPL, which stands for ubiquitin-proline-LacI, acts as a destabilizing module that permits rapid degradation of appended proteins. Image is Fig 7A, bottom panels, in J Cell Biol. (2011) 192: 599-614. Images in Fig 7 include CIL #13888, 13872, 13813, 13889, 13874.

• 2021

• Monje-Casas, Fernando
• Valerio-Santiago, Mauricio

Cells (MATa tem1::GAL-UPL-TEM1-TRP1 bfa1::HIS3 pRS316::eGFP-TEM1-CAAX) were fixed in 2.5% formaldehyde for 10 min, washed twice, and resuspended in 0.1 M potassium phosphate buffer, pH 6.4. Cells were then fixed for 10 min in 80% ethanol and resuspended in 1 mg/ml DAPI. Imaging was performed at 25C using a Leica DM6000 microscope equipped with a 100x/1.40 NA oil immersion objective lens, A4, L5, and TX2 filters, and a digital CCD camera (DFC350, Leica). Pictures were processed with LAS AF (Leica) and ImageJ software.

Preparation: formaldehyde fixed tissue; ethanol fixed tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: widefield illumination; fluorescence microscopy; differential interference contrast microscopy
Parameter imaged: fluorescence emission; optical path length gradient
Source of contrast: distribution of a specific protein; boundaries between regions with different refractive index
Visualization methods: EGFP
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation

• Cell Image Library Group ID: 9034

• Saccharomyces cerevisiae

• Cytoplasm
• Cytoskeleton
• Spindle pole body

• Cell cycle
• Cell division
• GTPase activator activity
• GTPase activity
• Mitosis
• Mitotic cell cycle spindle orientation checkpoint
• Nucleotide binding
• Protein binding
• Regulation of exit from mitosis
• Small GTPase mediated signal transduction
• W303

• data
• image

• No linguistic content; Not applicable

Samplenumber: 13872

Doi: https://doi.org/10.6075/J0251GX2

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL13872

Reference

• J Cell Biol. (2011) 192: 599-614. https://www.ncbi.nlm.nih.gov/pubmed/?term=21321099

Other resource

• BioStudies (previously JCB DataViewer): https://www.ebi.ac.uk/biostudies/studies/

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2022-08-12