Woodcock, Christopher (2021). CIL:713, Necturus maculosus, nucleate erythrocyte. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0MS3S0M

X-ray analysis shows that the nucleated erythrocytes of the amphibian Necturus maculosus (Mud Puppy) contains more ordered chromatin than most nuclei. Here, chromatin fibers were isolated from N. maculosus erythrocyte nuclei using mild micrococcal nuclease digestion, fixed briefly with glutaraldehyde, applied to thin carbon films and negatively stained with methylamine tungstate. ~30 nm fibers are seen, some exhibiting short regions with apparent structural order.

• 2021

• Woodcock, Christopher

Preparation: unembedded tissue; glutaraldehyde fixed tissue
Relation to intact cell: isolated subcellular component
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: elastic scattering
Visualization methods: other negative stain
Processing history: raw unprocessed data

• Necturus maculosus

• Nuclear chromatin
• Nucleate erythrocyte

• Chromatin assembly
• DNA packaging

• data
• image

• No linguistic content; Not applicable

Samplenumber: 713

Doi: https://doi.org/10.6075/J0MS3S0M

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL713

Reference

• PubMed article: https://www.ncbi.nlm.nih.gov/pubmed/?term=1757505
• Woodcock CL, Woodcock H, Horowitz RA. Ultrastructure of chromatin. I. Negative staining of isolated fibers. J Cell Sci. 1991 May;99 ( Pt 1):99-106. https://doi.org/10.1242/jcs.99.1.99

CC0 1.0 Universal Public Domain Dedication

Creative Commons Public Domain Dedication

Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.

Constraint(s) on Use: This work may be used without prior permission.

Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12