CIL:36751, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist
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- Collection
- Cite This Work
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Allen, Richard (2021). CIL:36751, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J09885ZN
- Description
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Phagoacidosomes are heavily labeled for L1 antigen in 5-8 to 15-18 minute-old DVs. However, only some DVs at 15-18 min still had L1 label. Only occasionally did we see tubules with label arising from the 15-18 min-old vacuole to support the idea that DV-II membrane is retrieved during the transformation of phagoacidosomes to phagolysosomes. The DV shown here was 15-18 min old as it contained 0.3µm latex beads. There is a small amount of DV-II specific L1 immunogold label in the tubules and in their bulbous ends. The phagolysosome membrane itself is totally free of this label. TEM taken on 2/18/92 by R. Allen with Zeiss 10A operating at 80kV. Neg. 12,000X. Bar = 0.5µm.
- Date Issued
- 2021
- Researcher
- Methods
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Glutaraldehyde fixation followed freezing and microtomy. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
- Technical Details
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Preparation: glutaraldehyde fixed tissue; tissue in vitreous ice embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; gold; uranyl salt
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed - Scientific Name
- Anatomy
- Topic
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- Language
- No linguistic content; Not applicable
- Identifier
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Samplenumber: 36751
- Related Resource
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL36751
Source data
- License
- Copyright
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Creative Commons Public Domain Dedication
Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Constraint(s) on Use: This work may be used without prior permission.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12