Svitkina, Tatyana M.; Borisy, Gary G. (2021). CIL:24783, Xenopus laevis, fibroblast. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0FX787W

Localization of XAC (Xenopus ADF/cofilin) in Xenopus fibroblasts. Fluorescence microscopy of a cell fragment double stained with XAC antibody (green) and TRITC-phalloidin (red). XAC is distributed throughout the entire lamellipodium. Image corresponds to Figure 10a-c from J Cell Biol. 1999 May 31;145(5):1009-26.

• 2021

• Borisy, Gary G.
• Svitkina, Tatyana M.

Procedures for detergent extraction, immunostaining, S1 decoration, light, and EM were described previously (Svitkina et al., 1995, 1996, 1997;Verkhovsky et al., 1995; Svitkina and Borisy, 1998).

Preparation: glutaraldehyde fixed tissue; permeabilized tissue; polyethylene glycol
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein; distribution of epitope
Visualization methods: Fluorescein (FITC); Tetramethyl rhodamine (TRITC); phalloidin; primary antibody plus labeled secondary antibody
Processing history: unprocessed raw data

• Cell Image Library Group ID: 9875

• Xenopus laevis

• Actin cytoskeleton
• Fibroblast
• Lamellipodium
• Xenopus ADF/cofilin

• Cellular localization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 24783

Doi: https://doi.org/10.6075/J0FX787W

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL24783

Reference

• J Cell Biol. 1999 May 31;145(5):1009-26. https://www.ncbi.nlm.nih.gov/pubmed/?term=10352018

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2022-08-12