Franzini-Armstrong, Clara; Loesser, Kathryn (2021). CIL:37397, damselfly, flight muscle cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0FF3RP5

Longitudinal and cross sections showing ribbon like myofibrils regularly alternating with layers of mitochondria and SR/T tubules. Dyads are located in a grove of the mitochondrial surface. Negatives aere scanned on nikon 8000 at 4000dpi/6.3 micron scan step size, then reduced in size 4X for upload, for an effective pixel size of 25 micron.

Technical details:
Heads and tail removed, thorax bisected and immersed in 3% glutaraldehyde buffered in 0.1 M cacodylate buffer (pH7.2). Stored at 4C for several days. Muscle dissected out. Rinsed 3Xin buffer, postfixed in 2% PsO4 in buffer, for 1.5 hrs; rinsed 3 times in water, en-block stained in saturated aqueous uranyl acetate, embedded in Spurr. Thin sections stained in uranyl acetate and lead and examined in a Philips 410 Microscope.

Reference for the image:
Loesser, K.E., Castellani, L. and Franzini-Armstrong, C. Disposition of junctional feet in muscles of invertebrates. J. Muscle Research Cell Motility 13:161-173, 199

• 2021

• Franzini-Armstrong, Clara
• Loesser, Kathryn

Preparation: glutaraldehyde fixed tissue; tissue in epoxy resin embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt; lead salt
Processing history: digitized negative film
Data qualification: Raw;spatialmeasurements

• Damselfly

• Flight muscle cell
• Mitochondrion
• Myofibril
• Sarcoplasmic reticulum
• T-tubule

• Membrane depolarization
• Mitochondrial proton-transporting ATP synthase complex assembly
• Sequestering of calcium ion
• Skeletal muscle contraction

• data
• image

• No linguistic content; Not applicable

Samplenumber: 37397

Doi: https://doi.org/10.6075/J0FF3RP5

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL37397

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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12