Muller, Waltraud G.; Haymann, J. Bernard; Nagashima, Kunio; Guttman, Peter; Werner, Stephan; Rehbein, Stefan; Schneider, Gerd; McNally, James G.; Heim, Stefan; Mueller, Florian (2021). CIL:41365, Mus musculus, adenocarcinoma. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J03T9G0V

The image shows a slice through a 3D tomographic reconstruction of a mouse adenocarcinoma cell based on x-ray microscopy. Live cells were cryofixed by plunge freezing in liquid ethane and the vitrified material observed unstained using x-ray microscopy at a wavelength that yields high contrast between water and organic material. Images were taken over a range of specimen tilts from -60 to +60 degrees at 1 degree intervals. The image represents a 15.6 nm thick slice of the computed tomogram in the x-y plane, and shows a nucleolus containing granular components of various size and density. The images have a resolution of ~36 nm (Rayleigh Criterion) and ~70 nm (Fourier Ring Correlation), the latter corresponding to ~35 nm half-pitch resolution. Additional X-ray images from the study are included in this CIL Group.
See also: W G Muller et al. 2012 Towards an atlas of mammalian cell ultrastructure by soft X-ray tomography. J Struct Biol 177:179-192.

• 2021

• Guttman, Peter
• Haymann, J. Bernard
• Heim, Stefan
• McNally, James G.
• Mueller, Florian
• Muller, Waltraud G.
• Nagashima, Kunio
• Rehbein, Stefan
• Schneider, Gerd
• Werner, Stephan

Mouse adenocarcinoma cells were grown on gold grids coated with cellulose nitrate, incubated with 270 nm diameter gold-coated silica beads, plunge frozen in liquid ethane, transferred to a Gatan 630 cryoholder, and imaged at -170C in the frozen hydrated state using the X-ray microscope at the Helmholz Zentrum, Berlin, Germany. The microscope uses partially coherent illumination and a zone plate objective, and allows the specimen to be observed over a range of tilts. The images were aligned using the gold beads as fiduciary markers, and reconstructed using a reciprocal space algorithm.
See: G Schneider et al. 2010 Three-dimensional cellular ultrastructure resolved by X-ray microscopy. Nat Meth 7:985-987.

Preparation: cryofixed tissue
Relation to intact cell: slice of 3D tomogram
Item type: recorded image
Imaging mode: X-ray micrograph
Parameter imaged: x-ray scattering
Source of contrast: intrinsic mass distribution
Visualization methods: visualization of contiguous regions
Data qualification: Processed

• Cell Image Library Group ID: 11959

• Mus musculus

• Adenocarcinoma
• Nucleolus
• Nucleus

• Nucleolus organization
• Nucleus organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 41365

Doi: https://doi.org/10.6075/J03T9G0V

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL41365

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2022-08-12