Allen, Richard (2021). CIL:10261, Halteria grandinella, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0GX49P9

An image of the buccal cavity of Halteria has oral ciliature on its left side and a paroral kinety consisting of only one row of basal bodies and cilia on its right side. A cytopharynx with microtubular lamellae arises to the right of the paroral kinety where it will continue deep into the cell as a cytopharyngeal tube accompanied by the microtubular lamellae. A thick layer of microtubules covers one side of the buccal cavity. Mitochondria are both expanded and condensed). TEM taken on 3/12/71 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 9,250X. The negative was printed to paper and the image was scanned with a flatbed scanner and the 0.5µm bar added in Photoshop. This digitized image is available for qualitative analysis. A raw, unprocessed, high resolution version of this image (CIL:7319) is in the library and available for quantitative analysis.
Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness.

Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

• 2021

• Allen, Richard

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; Print scanned for Photoshop
Data qualification: Processed

• Cell Image Library Group ID: 3456

• Halteria grandinella

• Cell by organism
• Ciliary rootlet
• Ciliated Protist
• Cytoskeleton
• Eukaryotic cell
• Eukaryotic Protist
• Oral apparatus

• Carolina Biological Supply Co., NC, U.S.A.
• Cytoskeleton organization
• Response to food

• data
• image

• No linguistic content; Not applicable

Samplenumber: 10261

Doi: https://doi.org/10.6075/J0GX49P9

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL10261

Other resource

• Paramecium and Other Ciliates: Richard Allen's Image Collection. Pacific Biosciences Research Center. University of Hawaii at Manoa: https://www5.pbrc.hawaii.edu/allen/

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2022-08-12