Huranova, M.; Ivani, I.; Benda, A.; Poser, I.; Brody, Y.; Hof, M.; Shav-Tal, Y.; Neugebauer, K.M.; Stanek, D. (2021). CIL:26584, Homo sapiens, cervical epithelium. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J09Z93SV

HeLa cells stably expressing BAC-encoded GFP-labeled snRNP protein hPrp4 and imaged witb a Leica SP5 confocal microscope using a 60x 1.4NA HCX Plan Apo objective lens. The hPrp4 is confined to the nucleoplasm and enriched in splicing factor compartments and Cajal Bodies (bright dots). From Fig 1A in Huranova et al. 2010 J Cell Biol 191:75-86.

• 2021

• Benda, A.
• Brody, Y.
• Hof, M.
• Huranova, M.
• Ivani, I.
• Neugebauer, K.M.
• Poser, I.
• Shav-Tal, Y.
• Stanek, D.

Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP
Processing history: frame averaging
Data qualification: Processed

• Cell Image Library Group ID: 10294

• Homo sapiens

• Cervical epithelium
• Pre-mRNA
• SnRNP
• Spliceosomal complex

• HeLa
• RNA metabolic process
• RNA splicing

• data
• image

• No linguistic content; Not applicable

Samplenumber: 26584

Doi: https://doi.org/10.6075/J09Z93SV

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL26584

Reference

• M Huranova et al. 2010 J Cell Biol 191:75-86 https://doi.org/10.1083/jcb.201004030

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2022-08-12