Hatano, Toshiyuki; Sluder, Greenfield (2021). CIL:34849, Homo sapiens, retinal pigment epithelium. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J02V2F18

Movie of cell growth and division in cultured hTERT-RPE1 cells (telomerase immortalized human retinal pigment epithelium) using DIC optics. This movie and the stack of time series images used to prepare them are grouped in the Cell Image Library.

• 2021

• Hatano, Toshiyuki
• Sluder, Greenfield

Cells were cultured on glass coverslips in 10% FBS, DMEM/F12 medium plus glutamine, HEPES, penicillin and streptomycin and examined with a Leica DMIRE2 microscope with Leica 100x HCX PLAN APO NA 1.35 DIC objective lens. images were captured at 5 sec intervals with a Hamamatsu ORCA-ER2 CCD camera using Simple PCI 6.6.0.0 software.

Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: differential interference contrast microscopy
Parameter imaged: optical path length gradient
Source of contrast: boundaries between regions with different refractive index
Visualization methods: visualization of contiguous regions
Processing history: unprocessed raw data

• Cell Image Library Group ID: 8515

• Homo sapiens

• Retinal pigment epithelium

• Cell proliferation
• HTERT-RPE1
• Mitosis

• data
• image
• video

• No linguistic content; Not applicable

Samplenumber: 34849

Doi: https://doi.org/10.6075/J02V2F18

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL34849

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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12