Korobova, Farida; Svitkina, Tatyana (2021). CIL:40659, Rattus, hippocampal neuron. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0GQ6WNK

Platinum replica depicting the cytoskeletal organization of dendritic spines in extracted 14 DIV neurons. Mushroom spines associate with dendrites at the base (bottom) and with axons by the head (top). Thick fibers in neurites represent microtubules. Image corresponds to Figure 2a in Mol Biol Cell. 2010 Jan 1;21(1):165-76. The entire panel is available as CIL 40660. The uncolorized image (this image) is available as CIL 40659. Box 1, 2, and 3 are CIL 40661, 40662, and 40663, respectively.

• 2021

• Korobova, Farida
• Svitkina, Tatyana

Please refer to referenced article for details on cell extraction and processing for electron microscopy.

Preparation: detergent permeabilized; glutaraldehyde fixed tissue; critical_point dried specimen
Relation to intact cell: dispersed cells in vitro
Item type: grey scale graphic
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in deposition of metal shadow
Visualization methods: shadowing and plating
Processing history: unprocessed raw data

• Cell Image Library Group ID: 11349

• Rattus

• Actin cytoskeleton
• Dendritic shaft
• Dendritic spine
• Hippocampal neuron
• Intermediate filament cytoskeleton
• Microtubule cytoskeleton
• Post synaptic density

• Dendrite morphogenesis
• Dendritic spine organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 40659

Doi: https://doi.org/10.6075/J0GQ6WNK

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL40659

Reference

• Korobova F, Svitkina T. Molecular architecture of synaptic actin cytoskeleton in hippocampal neurons reveals a mechanism of dendritic spine morphogenesis. Mol Biol Cell. 2010 Jan 1;21(1):165-76. https://doi.org/10.1091/mbc.e09-07-0596
• Mol Biol Cell. 2010 Jan 1;21(1):165-76. Epub 2009 Nov 4. https://www.ncbi.nlm.nih.gov/pubmed/?term=19889835

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2022-08-12