Ivanchenko, Maryna V.; Cicconet, Marcelo; Jandal, Hoor Al; Wu, Xudong; Corey, David P.; Indzhykulian, Artur A. (2022). CIL: 50721, FIB-SEM Dataset of anti-PKHD1L1 Immuno-Gold Labeled Outer Hair Cell Stereocilia Bundles: 6.3_Cell_6_Stereocilia_Segmentation. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J01J995N

FIB-SEM Dataset of anti-PKHD1L1 Immuno-Gold Labeled Outer Hair Cell Stereocilia Bundles. Postnatal day 4 mouse Inner Ear Organ of Corti labeled with anti-PKHD1L1 antibody (10 nm gold beads). The dataset represents the apical end of the Outer Hair Cell with the stereocilium bundle. Images were acquired with FEI Helios FIB-SEM, using backscatter detector from an epoxy resin embedded inner ear Organ of Corti sample.

• 2022

• Cicconet, Marcelo
• Corey, David P.
• Indzhykulian, Artur A.
• Ivanchenko , Maryna V.
• Jandal, Hoor Al
• Wu, Xudong

Images were acquired with FEI Helios FIB-SEM, using backscatter detector from an epoxy resin embedded inner ear Organ of Corti sample.
6.3_Cell_6_Stereocilia_Segmentation is a multipage TIFF file illustrating stereocilia segmentation results. Same as ‘Towers.am’ file used for MATLAB volume analysis of this cell, but converted to TIFF to allow for its online preview. File contains 324 images, as all the planes that contained no segmentation data were removed.
This file is a part of Dataset 6, comprised of files related to the Cell #6 of current study. The numbering order of the files reflects the order of operations performed during the analysis. The raw imaging data related to this cell was deposited within Dataset 1 (CIL:50680).

Imaging mode: Serial Electron Microscopy; Focused Ion Beam Scanning Electron Mictoscopy (FIB-SEM)
Visualization methods: Heavy metal stain (Osmium Tetroxide, Potassium Ferrocyanide, Tannic Acid); Immunogold labeling with 10 nm gold beads

• Cell Image Library Group ID: 20006

• Mus musculus

• Cuticular plate
• Hair cell stereocilia
• Inner ear outer hair cell
• Mitochondria
• Polycystic Kidney and Hepatic Disease 1-Like 1 (PKHD1L1) labeling (gold beads)

• Native auditory Hair Cells at postnatal day 4

• data
• image

• No linguistic content; Not applicable

Samplenumber: 50721

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL50721

Reference

• Nature Communications article: https://www.nature.com/articles/s41467-019-11712-w

Creative Commons Attribution 4.0 International Public License

• UC Regents

Under copyright (US)

Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.

Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.

Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-11-18