Allen, Richard (2021). CIL:36258, Vorticella microstoma, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0MP51ZS

Freeze fracture of the plasma membrane and outer alveolar membrane of the pellicle of V. microstoma. The surface of Vorticella is encircled by rings which follow the septa of underlying alveoli. In this fracture one can see the fractured necks of the continuities between the outer and inner alveolar membranes (latter not visible). The outer alveolar membrane has many prominent IMPs on its P-fracture face. Septal pores extend between the continuities, thus the alveoli are all interconnected. The E-fracture face of the plasma membrane has only a moderate number of prominent IMPs compared to its P face. TEM taken on 11/11/75 by R. Allen with JEM 100B operating at 80kV. Neg. 14,579X. Bar = 0.25µm. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. There is a high resolution version of this image in the library (CIL:39325) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

• 2021

• Allen, Richard

Preparation: freeze_fracture/freeze_etch
Relation to intact cell: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: electron density
Parameter imaged: elastic scattering of electrons; electron density; elevation
Source of contrast: differences in deposition of metal shadow
Visualization methods: transmission electron microscopy (TEM)
Processing history: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film; Print of negative scanned for Photoshop
Data qualification: Processed

• Cell Image Library Group ID: 10337

• Vorticella microstoma

• Cell by organism
• Cell cortex
• Cell cortex part
• Ciliated Protist
• Eukaryotic cell
• Eukaryotic Protist
• Pellicle
• Plasma membrane

• Plasma membrane organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 36258

Doi: https://doi.org/10.6075/J0MP51ZS

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL36258

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2022-08-12