Rusielewicz, Tomasz; Melendez-Vasquez, Carmen (2021). CIL:40361, Rattus norvegicus, oligodendrocyte. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0639P4T

This fluorescent micrograph is part of an image group displaying the morphological changes associated with a developing oligodendrocyte. The progression of images starts with CIL:40358 where cells display a relatively bipolar morphology to CIL:40359 where the actin cytoskeleton becomes very prominent to CIL:40360 where the actin coalesces and interlinks and finally there is CIL:40361 where one can observe the formation of a flattened lamellar structure which in vitro mimics the myelin sheet.

• 2021

• Melendez-Vasquez, Carmen
• Rusielewicz, Tomasz

Rattus norvegicus oligodendrocytes purified from P2 cortex. Cells were fixed on glass coverslips with 4% PFA and immunostained with Phalloidin-FITC (green), alpha-tubulin primary ab./Rhodamine secondary ab (red), RIP (oligodendrocyte marker) primary ab/Cy5 secondary ab (blue), and DAPI (nuclear marker also in blue). Photographed using Leica DMI4000B microscope and 63x HCX PL APO OIL Objective. Voxel size 0.1um.

Preparation: formaldehyde fixed tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: phalloidin; primary antibody plus labeled secondary antibody; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: unprocessed raw data

• Cell Image Library Group ID: 11012

• Rattus norvegicus

• Actin cytoskeleton
• Microtubule
• Nucleus
• Oligodendrocyte
• RNA Immunoprecipitation (RIP)

• Oligodendrocyte development

• data
• image

• No linguistic content; Not applicable

Samplenumber: 40361

Doi: https://doi.org/10.6075/J0639P4T

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL40361

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2022-08-12