Murray, John; Hu, Ke (2021). CIL:10545, Toxoplasma gondii RH. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J05D8R1Q

A monolayer of human foreskin fibroblast cells was infected with Toxoplasma gondii expressing YFP-tagged TgIMC1 (innermembrane complex scaffold protein) and mCherry-tagged human alpha1tubulin. Images acquired using API DeltaVision on a Olympus IX70 inverted microscope. Objective: Olympus 60X water immersion, 1.2 NA. Filters: mCherry, ex 555-592 em 600-665, YFP, ex 490-510 em 525-595. Captured with CoolSnapHQ/ICX285. Image stack corresponds to deconvolved stack: CIL# 10546, maximum projection stack: CIL# 10547 and raw data stack with DIC: CIL# 10542.

Unpublished image that is similar to images published in PMID: 16518471. See publication for other experimental details.

• 2021

• Hu, Ke
• Murray, John

Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image; charge coupled device (CCD)
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: Yellow fluorescent proteins from Aequorea; mCherryFP
Data qualification: Raw;spatialmeasurements;intensitiesquantitation

• Cell Image Library Group ID: 5765

• Toxoplasma gondii RH

• Centriole
• Conoid
• Cortical microtubule
• Inner membrane complex
• Spindle pole

• Cell division
• Microtubule cytoskeleton organization
• Regulation of cell shape
• Structural constituent of cytoskeleton
• Toxoplasma gondii RH stable cell line
• Toxoplasmosis

• data
• image

• No linguistic content; Not applicable

Samplenumber: 10545

Doi: https://doi.org/10.6075/J05D8R1Q

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL10545

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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12