Aihara, M.; Allen, Richard (2021). CIL:7666, Nassula, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0CC0ZR3

Higher resolution image of the surface of Nassula aurea. Early endosomes and clathrin coated vesicles. One clathrin coated pit at the bottom of a parasomal sac is shown. TEM taken on 2/22/71 by M. Aihara with Hitachi HU11A operating at 50kV. Neg. 10,750X. The raw film was scanned with an Epson Perfection V750 Pro. This image is available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm.

Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

• 2021

• Aihara, M.
• Allen, Richard

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation

• Cell Image Library Group ID: 4832

• Nassula

• Cell by organism
• Cell cortex
• Ciliated Protist
• Clathrin coat
• Eukaryotic cell
• Eukaryotic Protist
• Parastomal sac

• Cambridge Culture Collection, Cambridge, England, catalog # LB1650/1
• Clathrin coat assembly
• Cortical cytoskeleton organization
• Microtubule cytoskeleton organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 7666

Doi: https://doi.org/10.6075/J0CC0ZR3

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL7666

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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12