Cammer, Michael; Novikoff, Phyllis (2021). CIL:35280, Rattus rattus, hepatocyte. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0C24VCB

Primary rat hepatocytes in culture fixed and stained for beta-tubulin. The microtubules form a network throughout the cytoplasm. In this image they are seen originating from a brighter and denser region adjacent to the cell nucleus.

This is a single optical section a few microns up from the substrate collected by widefield fluorescence microscopy with a Photometrics camera with a KAF1400 chip. The imaging was performed at a microscopy course at Woods Hole Oceanographic Institute in October 1993 and deconvolved on VayTec software running on a Macintosh computer. At the time, deconvolution was new and novel for use by biologists.

• 1993-10-01

• 2021

• Cammer, Michael
• Novikoff, Phyllis

Image was collected with 1 60X, 63X, or 100X oil immersion objective with a numerical aperture of 1.25 such that each pixel size was 0.23 X 0.23 um. The image was collected as part of a Z series with step size of 0.53 um. A Photometrics cooled CCD camera based on the KAF1400 chip was used.

Preparation: formaldehyde fixed tissue; glutaraldehyde fixed tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: Rhodamine; primary antibody plus labeled secondary antibody
Processing history: 3D-deconvolution
Data qualification: Processed;spatialmeasurements

• Cell Image Library Group ID: 8536

• Rattus rattus

• Cytoplasmic microtubule
• Hepatocyte
• Microtubule organizing center
• TS25,liver

• Interphase

• data
• image

• No linguistic content; Not applicable

Samplenumber: 35280

Doi: https://doi.org/10.6075/J0C24VCB

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL35280

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2023-08-17