LaFountain, James R.; Oldenbourg, Rudolf (2021). CIL:12369, Nephrotoma, Nephrotoma suturalis, primary spermatocyte. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0348JK1

Movie showing showing the dynamics of kinetchore microtubules during meiosis I in primary spermatocytes of the crane-fly Nephrotoma suturalis that were experimentally flattened. Time-lapse polarization microscopy using a Nikon Microphot SA, equipped for liquid crystal polarized light microscopy
(LC-PolScope, CRi, Woburn Massachusetts) 60x/1.4 PlanApo oil immersion objective, 1.4 NA oil imm. condenser, with 2.0x zoom lens. Images captured every 15 sec using a QImaging Retigo EXi CCD camera. Raw images were processed
using 5-frame algorithm (Shribak and Oldenbourg, 2003). The time series used for the movie is included in this grouped set.

• 2021

• LaFountain, James R.
• Oldenbourg, Rudolf

Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD); Processed image based on 5 recorded images
Imaging mode: polarization microscopy
Parameter imaged: UV/visible/IR photons
Source of contrast: intrinsic birefringence
Visualization methods: relative retardation (retardance) measurement
Processing history: 5-frame algorithm

• Cell Image Library Group ID: 3779

• Nephrotoma
• Nephrotoma suturalis

• Nuclear chromosome
• Primary spermatocyte
• Spindle

• Male meiosis chromosome segregation

• data
• image
• video

• No linguistic content; Not applicable

Samplenumber: 12369

Doi: https://doi.org/10.6075/J0348JK1

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL12369

Reference

• LaFountain JR, Oldenbourg R (2004) Mol Biol Cell 15, 5346-5355. https://doi.org/10.1091/mbc.e04-06-0524
• Oldenbourg R, Mei G (1995) J Microsc 180, 140-147
• PubMed article: https://www.ncbi.nlm.nih.gov/pubmed/?term=12790452
• PubMed article: https://www.ncbi.nlm.nih.gov/pubmed/?term=8537959
• Shriback M, Oldenbourg R (2003) Appl Opt 42, 3009-3017 https://www.ncbi.nlm.nih.gov/pubmed/?term=469981
• Shribak M, Oldenbourg R. Techniques for fast and sensitive measurements of two-dimensional birefringence distributions. Appl Opt. 2003 Jun 1;42(16):3009-17. https:/doi.org/10.1364/ao.42.003009

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2022-08-12