Microscopy product ID: 3428
2D Image
Description | Zero tilt image through a 0.25 um section of blue green algae exposed to high light conditions imaged on an intermediate voltage electron microscope at 400 KeV. Contrast is reversed and adjusted compared to original data. While spots are colloidal gold particles applied to the surface to server a fiduciary cues. Full resolution data can be viewed by clicking on the Neuroinformatica link. |
Embed
Embed URL
Embed Image
Full resolution data file
File Size |
|
File Format |
|
Description | Zip file containing compressed unaligned digitized 2D data in suprim format (*.f.gz) along with the fiducial mark file generated by xfido (.fido) and a file containing the angles for each image generated by saxalign (.ang) |
Segmentation
Description | Manual segmentation of thylakoid membranes and other cellular structures using Xvoxtrace 2.9, followed by surfacing using the nuages algorithm and Synu. |
Embed
Embed URL
Embed Image
Full resolution data file
File Size |
|
File Format |
|
Description | Tar file containing the original trace file (phaeo23.trace), along with the surface files for each object (*.synu) and the Viewdata file required to view them using Synu. |
Reconstruction
Description | Computed slice through a tomographic reconstruction of the chloroplast and thylakoid membranes from a blue green algae grown in high light conditions. |
Embed
Embed URL
Embed Image
Full resolution data file
File Size |
|
File Format |
|
Description | Tar file containing volume in Analyze 7.5 format. File contains both .hdr and .img file. This file was resectioned obliquely to flatten out the section in the XY plane so it is degraded slightly in resolution from the original volume. |
Animation
Embed
Embed URL
Embed Video
Description | Animation through computed slices of the tomographic reconstruction of blue green algae grown in high light conditions. |
Full metadata
- Collection
- Cite This Work
-
Moisan, Tiffany; Sosinsky, Gina; Buitenhuys, Casey; Ellisman, Mark H. (2017). Microscopy product ID: 3428. In Cell Centered Database. UC San Diego Library Digital Collections. https://doi.org/10.6075/J0XS5V57
- Date Issued
- 2017
- Research Team Head
- Researchers
- Technical Details
-
Product type: SINGLE TILT. Microscopy type: IVEM. Instrument: JEOL 4000EX IVEM
Strain: Karsten
- Funding
-
National Aeronautics and Space Administration
- Series
- Scientific Name
- Anatomy
- Topic
Formats
View formats within this collection
- Language
- No linguistic content; Not applicable
- Related Resources
- Moisan TA, Ellisman MH, Buitenhuys CW, Sosinsky GE (2006). Differences in chloroplast ultrastructure of Phaeocystis antarctica in low and high light. Marine Biology, 149(6):1281-1290. https://doi.org/10.1007/s00227-006-0321-5
- Microscopy product 3428 at the Cell Centered Database: https://doi.org/10.7295/W9CCDB3428
Primary associated publication
Other version
- License
-
Creative Commons Attribution 4.0 International Public License
- Rights Holder
- UC Regents
- Copyright
-
Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
- Digital Object Made Available By
-
Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2023-05-22