Mettlen, Marcel; Schmid, Sandra L. (2021). CIL:10105, Homo sapiens, epithelial cell, retinal pigmented epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J04B30DN

Human retinal pigmented epithelial (RPE) cells labeled for clathrin-coated pits (green), focal adhesions (red) and nuclei (blue). RPE cells stabily expressing 'clathrin light chain a' tagged with EGFP (EGFP-LCa) were plated directly on glass coverslides. After 72 hrs, cells were fixed for 2 min in a 1:1 mixture of 2% paraformaldehyde/0.5% Triton X-100, followed by a 30 min fixation in 4% paraformaldehyde. Cells were then stained with mouse monoclonal antibodies to paxillin, detected with goat anti-mouse Alexa 568. Nuclei were stained with DAPI. Image was collected on an Olympus IX71 inverted microscope coupled to a Hamamatsu ORCA-ER digital camera using a 60x 1.42 NA Plan Apo N objective (Olympus). The exposure times were 200, 500 and 70 msec for the red, green and blue channel, respectively. A xenon-arc lamp (Lambda LS stand-alone illuminator; Sutter Instrument) was used with adequat filters (Chroma). Bar: 5µm

• 2021

• Mettlen, Marcel
• Schmid, Sandra L.

Preparation: formaldehyde fixed tissue; detergent permeabilized
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP; Alexa Fluor 568; 4',6-diamidino-2-phenylindole (DAPI); primary antibody plus labeled secondary antibody
Processing history: 3 color combine

• Homo sapiens

• Clathrin coat of coated pit
• Clathrin light chain a
• Epithelial cell
• Focal adhesion
• Nucleus
• Retinal pigmented epithelial cell

• data
• image

• No linguistic content; Not applicable

Samplenumber: 10105

Doi: https://doi.org/10.6075/J04B30DN

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL10105

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2022-08-12