Sun, Yuting; Ge, Yejing; Drnevich, Jenny; Zhao, Yong; Band, Mark; Chen, Jie (2021). CIL:13593, Mus musculus, myoblast. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J09885RZ

The C2C12 cell line, a mouse myoblast line, was used here to study the regulatory factors in myogenic differentiation. After cultured in differentiation medium for 3 days, these cells differentiated into myotubes (Green) containing multiple nuclei (Blue). The C2C12 cells stably expressing FLAG-tagged rapamycin-resistant, kinase-inactive (RR/KI) mTOR were induced to differentiate in the presence of 50nM rapamycin (Rap) for 3 days. 25nM TSA was added when the cells were ~60% confluent and removed the next day upon induction of differentiation. TSA was able to rescue the effect of Rap to allow myotube maturation.

This image is the control of different treatments in Figure 9B from JCB 189: 1157-1169, 2010. See also CIL: 13592, 13594, 13601.

• 2021

• Band, Mark
• Chen, Jie
• Drnevich, Jenny
• Ge, Yejing
• Sun, Yuting
• Zhao, Yong

The image was taken by leica DMI 4000B microscope equipped with a QImaging RETIGA Exi camera and Leica 10X/0.22 lens.

Myosin heavy chain (MHC) expressed in myotubes was labeled by MF-20 mouse antibody followed by FITC-anti-mouse IgG secondary antibody. Nuclei were stained by DAPI. MHC and nuclei were pseudocolored in green and blue, respectively.

Preparation: formaldehyde fixed tissue; permeabilized tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: compartmentalization of stain or label; distribution of epitope
Visualization methods: Fluorescein (FITC); 4',6-diamidino-2-phenylindole (DAPI); primary antibody plus labeled secondary antibody
Processing history: brightness and contrast adjusted
Data qualification: Processed;spatialmeasurements;intensitiesquantitation

• Cell Image Library Group ID: 8288

• Mus musculus

• Muscle myosin complex
• Myoblast
• Nucleus

• C2C12
• Histone deacetylase inhibitor activity
• MTOR inhibition
• Myoblast fusion

• data
• image

• No linguistic content; Not applicable

Samplenumber: 13593

Doi: https://doi.org/10.6075/J09885RZ

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL13593

Reference

• JCB 189: 1157-1169, 2010 https://www.ncbi.nlm.nih.gov/pubmed/?term=20566686

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2022-08-12