Withers, Ginger (2021). CIL:843, Rattus, multipolar neuron. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J08P60J8

CFP-expressing neuron, 14 days in vitro. By two weeks, cultured hippocampal neurons have developed a morphology typical of mature neurons with an extensive axonal arbor, a tapered and branched dendritic arbor, and synaptic connections. Growth cones can be seen at the tips of axonal branches, and the dendritic arbor is studded with spines, specialized structures commonly associated with excitatory synapses. This image accompanies the second file in this image group which shows the fluorescent image.

Embryonic rat hippocampal neurons were prepared and transfected with soluble CFP (excitation, 439; emission, 476) at plating (Dotap, Roche) as previously described (Kaech and Banker, 2006, Nat Protoc), fixed for 15 minutes (4% formaldehyde, 4% sucrose in phosphate buffered saline, pH 7.4, warmed to 37°C, permeabilized with 0.25% Triton X-100 for 7 minutes, and rinsed with phosphate buffered saline at 14 days in vitro (Withers and Banker, 1998, in Culturing Nerve Cells, MIT Press). Images were acquired with a Leica DMRXA microscope with a mercury arc lamp, a 16X PL Fluotar (0.5 NA) oil immersion lens, Princeton Instruments Micromax CCD camera and MetaMorph software, using a Leica CFP filter cube. Linked images show: A) CFP expression throughout the extent of the neuron; B) a merged image of the fluorescent cell with respect to the phase image. This image was generated with the MetaMorph overlay function, and the single plane fluorescence view is included in this image group.

• 2021

• Withers, Ginger

Preparation: formaldehyde fixed tissue; detergent permeabilized
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy; phase contrast microscopy
Parameter imaged: fluorescence emission; refractive index
Source of contrast: in-focus phase contrast; Accumulation of fluorescent probe
Visualization methods: Cyan fluorescent proteins from Aequorea

• Cell Image Library Group ID: 7904

• Rattus

• Axon
• Dendrite
• Growth cone
• Multipolar neuron
• Neuron spine

• Cellular developmental process
• Dendrite development
• Establishment or maintenance of cell polarity

• data
• image

• No linguistic content; Not applicable

Samplenumber: 843

Doi: https://doi.org/10.6075/J08P60J8

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL843

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2022-08-12