CIL:36714, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist
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- Collection
- Cite This Work
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Allen, Richard (2021). CIL:36714, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0SJ1JGC
- Description
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Two of the three types of vesicles that are transported to the cytopharynx and that accumulate in large numbers at the cytopharynx are shown in this electron micrograph. The discoidal vesicles move up to the edge of the cytopharynx membrane where the cytopharynx membrane is continuous with the plasma membrane of the buccal cavity. Here the discoidal vesicles fuse with the cytopharynx membrane. Just behind this edge is found a layer of larger vesicles, the acidosomes, which dock with the cytopharynx membrane as soon as they make contact with this membrane. Unlike the discoidal vesicles these acidosomes do not fuse with the cytopharynx or nascent vacuole until the phagosome has pinched off and moved toward the posterior pole of the cell. TEM taken on 4/8/83 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 8,000X. Bar = 0.5µm.
- Date Issued
- 2021
- Researcher
- Methods
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After a light glutaraldehyde fixation, these organisms were incubated in Gomori reagent to reveal the presence of acid phosphatase then dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
- Technical Details
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Preparation: glutaraldehyde fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed - Scientific Name
- Anatomy
- Topic
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- Language
- No linguistic content; Not applicable
- Identifier
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Samplenumber: 36714
- Related Resource
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL36714
Source data
- License
- Copyright
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Creative Commons Public Domain Dedication
Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Constraint(s) on Use: This work may be used without prior permission.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12