Cell Image Library (2021). CIL:36023, Chlorocebus aethiops, permanent cell line cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J03X85B8

COS7 cells, cotransfected with GFP-PTP1B, APN-EphA3, and TM-BirA, were stimulated with SA Dynabeads. Cell surface EphA3 was labeled on intact cells with IIIA4 α-EphA3 mAb and detected with Alexa Fluor 546–labeled secondary antibodies by confocal microscopy. PTP1B/EphA3 colocalization is apparent around SA Bynabeads.

• 2021

Preparation: chemically fixed tissue
Relation to intact cell: whole mounted tissue
Item type: fluorescence microscopy
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: Green fluorescent proteins from Aequorea; Alexa Fluor 546

• Cell Image Library Group ID: 8847

• Chlorocebus aethiops

• Permanent cell line cell

• COS-7
• Negative regulation of insulin receptor signaling pathway
• Protein tyrosine kinase activity

• data
• image

• No linguistic content; Not applicable

Samplenumber: 36023

Doi: https://doi.org/10.6075/J03X85B8

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL36023

Reference

• PubMed article: https://www.ncbi.nlm.nih.gov/pubmed/?term=21135139

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2022-08-12