Harding, Mark (2021). CIL:47302, Staphylococcus aureus, neutrophil, endothelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0F47MPX

Visualization of neutrophil recruitment in capillaries proximal to agarose beads. (Figure 4 - Perfusion experiments)

• 2021

• Harding, Mark

Mice were anesthetized with ketamine/xylazine, and then subcutaneous tissue on a dorsal flap was exteriorized. GFP staphylococcus aureus beads were inserted. Antibodies were injected into the vasculature to label neutrophils (red) and endothelial cells (blue). Blocking antibodies against the molecule Mac1 were administered after 2 hours of infection.

Relation to intact cell: intact cells-in vivo imaging
Item type: recorded image
Imaging mode: spinning disk confocal microscopy
Processing history: Converted from volocity to .avi

• Cell Image Library Group ID: 16611

• Staphylococcus aureus

• Endothelial cell
• Neutrophil

• Administration of FITC Albumin to identify blood flow through capillaries
• Occlusion of Microvasculature

• data
• image

• No linguistic content; Not applicable

Samplenumber: 47302

Doi: https://doi.org/10.6075/J0F47MPX

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL47302

CC0 1.0 Universal Public Domain Dedication

Creative Commons Public Domain Dedication

Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.

Constraint(s) on Use: This work may be used without prior permission.

Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12