Kizilyaprak, Caroline; Spehner, Danièle; Devys, Didier; Schultz, Patrick (2021). CIL:39745, Mus musculus, retinal rod cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0WH2P9R

TEM image of epon section of a freeze-substituted mouse retina illustrating the unusual distribution of heterochromatin in rod receptor nuclei where the compact heterochromatin is centrally located. Within the heterochromatin, two regions were identified, and inner 'smooth' mass (smo), and an outer 'granular compartment (gra), each with a distinct optical diffraction pattern (see inserts).
See Fig 2E in: C Kizilyaprak et al. 2010. In Vivo Chromatin Organization of Mouse Rod Photoreceptors Correlates with Histone Modifications. PLOS one 5:e11039

• 2021

• Devys, Didier
• Kizilyaprak, Caroline
• Schultz, Patrick
• Spehner, Danièle

Discs were punched from freshly dissected mouse retina, cryoprotectant added, the samples cryo-fixed using high pressure freezing using a Leica EMPACT2, freeze-substituted in acetone containing OsO4, and embedded in Epon. Sections were stained with uranyl acetate and lead citrate, and observed with a Tecnai F20 TEM, and tilt series recorded with a 2k CCD camera.
See also: C Kizilyaprak et al. 2010. In Vivo Chromatin Organization of Mouse Rod Photoreceptors Correlates with Histone Modifications. PLOS one 5:e11039

Preparation: cryofixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: charge coupled device (CCD)
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt; lead salt; osmium tetroxide
Data qualification: Processed

• Cell Image Library Group ID: 10713

• Mus musculus

• Chromatin
• Heterochromatin
• Nucleus
• Retinal rod cell

• Chromatin organization
• DNA packaging
• Photoreception

• data
• image

• No linguistic content; Not applicable

Samplenumber: 39745

Doi: https://doi.org/10.6075/J0WH2P9R

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL39745

Reference

• C Kizilyaprak et al. 2010. In Vivo Chromatin Organization of Mouse Rod Photoreceptors Correlates with Histone Modifications. PLOS one 5:e11039 https://www.ncbi.nlm.nih.gov/pubmed/?term=20543957

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2022-08-12