CIL:39163, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist
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- Collection
- Cite This Work
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Allen, Richard (2021). CIL:39163, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0FF3R06
- Description
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The left edge of the cytopharynx is bordered by a complex architecture designed to transport a pool of flattened vesicles to this edge where they will fuse with the cytopharyngeal membrane to expand this membrane into a nascent phagosome. The buccal cavity itself has the normal pellicle composed of the plasma membrane and alveoli. Internal to this is a system of regularly shaped electron opaque nodes that are bound together by whispy filaments to form a reinforced border. This is part of the filamentous reticulum which is found to a greater or lesser extent underlying the alveolar system of the entire buccal cavity but not the cytopharynx itself. Arising from the nodes are the cytopharyngeal microtubular ribbons that are spaced at regular intervals. These ribbons, composed of about 12 microtubules each, curve into the cytosol over a bundle of filaments called the cytostomal cord which is an extension of the centrin-containing infraciliary lattice. Numerous discoidal vesicles are bound to one side of the microtubules. Associated with the cytosomal cord are regularly spaced tubular extensions of the alveolar sacs that are connected by short fibers to the cord. TEM taken on 3/30/73 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 9,250X. Published in part in J. Cell Biol. 1974. 63:904-22. Adapted with permission.
- Date Issued
- 2021
- Researcher
- Methods
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Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
- Technical Details
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Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation - Series
- Scientific Name
- Anatomy
- Topics
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- Language
- No linguistic content; Not applicable
- Identifier
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Samplenumber: 39163
- Related Resources
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL39163
- Allen RD. Food vacuole membrane growth with microtubule-associated membrane transport in Paramecium. J Cell Biol. 1974 Dec;63(3):904-22. https://doi.org/10.1083/jcb.63.3.904
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Reference
- License
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License
- Rights Holder
- UC Regents
- Copyright
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Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12