CIL:12073, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist
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- Collection
- Cite This Work
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Allen, Richard (2021). CIL:12073, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0HT2NHQ
- Description
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A high resolution micrograph of the transverse microtubular ribbon, consisting of ~3 microtubules, extends to the cell's left of the basal body and rises to the top of the ridge where it ends. Thus the proximal end of a pair of basal bodies is firmly anchored by this “tripod” of accessory structures, the kd, pc mt and tr mt. Presumably this tripod will resist the torque induced on the basal bodies by the movements of the ciliary beat and hold the proximal ends of the basal body in a relatively rigid position. Septa of the alveolar sac system and septal pores are seen in this micrograph. Internally extruded trichocysts also seen in this micrograph. TEM taken on 1/12/73 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 9,900X. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
- Date Issued
- 2021
- Researcher
- Technical Details
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Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements - Series
- Scientific Name
- Anatomy
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- No linguistic content; Not applicable
- Identifier
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Samplenumber: 12073
- Related Resources
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL12073
- Paramecium and Other Ciliates: Richard Allen's Image Collection. Pacific Biosciences Research Center. University of Hawaii at Manoa: https://www5.pbrc.hawaii.edu/allen/
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- License
- Copyright
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Creative Commons Public Domain Dedication
Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Constraint(s) on Use: This work may be used without prior permission.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12