Svitkina, Tatyana M.; Verkhovsky, Alexander B.; Borisy, Gary G. (2021). CIL:23043, Rattus, fibroblast. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0Z31XHF

Plectin sidearms on intermediate filaments labeled with plectin immuno-gold (10 nm) in REF-52 cells with actin removed by gelsolin treatment. Untreated control appears as CIL: 234044. Electron microscopy of cytoskeletons was performed as described (Svitkina et al., 1995). Briefly, cells on coverslips were lysed as for light microscopy, treated, with recombinant gelsolin NHz-terminal domain, fixed with glutaraldehyde, tannic acid and uranyl acetate, critical point dried, and coated with platinum and carbon. Image corresponds to Fig 1c in J Cell Biol. 1996 Nov;135(4):991-1007.

• 2021

• Borisy, Gary G.
• Svitkina, Tatyana M.
• Verkhovsky, Alexander B.

Preparation: glutaraldehyde fixed tissue; critical_point dried specimen; tannic acid
Relation to intact cell: platinum replica
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in deposition of metal shadow
Visualization methods: uranyl salt; shadowing and plating; gold; primary antibody plus labeled secondary antibody; immunogold
Processing history: unprocessed raw data

• Cell Image Library Group ID: 8924

• Rattus

• Fibroblast
• Intermediate filament
• Plectin

• Gelsolin treatment
• REF-52

• data
• image

• No linguistic content; Not applicable

Samplenumber: 23043

Doi: https://doi.org/10.6075/J0Z31XHF

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL23043

Reference

• J Cell Biol. 1996 Nov;135(4):991-1007 https://www.ncbi.nlm.nih.gov/pubmed/?term=8922382

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2022-08-12